摘要:本文就富鋅香菇菌絲體的不同溶劑提取物對氧自由基、羥自由基的抑制能力和T-SOD活力進行了測定。

　　　100μg/mL的鋅對香菇菌絲體生長有促進的作用，富鋅香菇提取物的生物活性比未富鋅香菇提取物的生物活性高。通過微波消解-原子吸收法測定有機鋅的含量。

　　　利用T-SOD試劑盒以及羥自由基試劑盒試劑進行總SOD活力，羥自由基抑制能力的測定。經過數據分析得，富鋅香菇提取物的萃取時間越長，對氧自由基抑制率越高，對羥自由基抑制能力越強，總SOD活力越大。當萃取時間為30分鐘時，富鋅香菇水提取物對氧自由基抑制率最高為44.53％，且總SOD活力最大為434.57（U/ml）。當萃取時間為30分鐘時，富鋅香菇乙醇提取物對羥自由基抑制能力最高為392.79（U/ml）。

關鍵詞：香菇；富鋅；抗氧化；有機鋅

Abstract:This paper researched the clearing ability of different extracts of zinc-rich mushroom mycelium on oxygen free radicals and hydroxyl radicals and on T-SOD activity.

　　　The 100μg/mL zinc promote the growth of shiitake fungus , the biological activity of than the non-zinc-rich mushroom extract is higher than  the biological activity of the no zinc-rich one. The determination of organic zinc content is by Microwave Digestion - Atomic Absorption Spectrometry .

    The determination of activity of SOD and the hydroxyl radical suppression ability is by T-SOD kit and hydroxyl radical kit reagents . After data analysis , the longer the extraction time of the zinc-rich mushroom extract, the higher the rate of O2-radical scavenging, the stronger hydroxyl radical scavenging and the greater the activity of SOD. When the extraction time of 30 minutes, the O2- radical inhibition rate and the total SOD activity of the zinc-rich mushroom water extract  is highest ,which is respectively up to 44.53% and 434.57 U / ml. When the extraction time of 30 minutes, the hydroxyl radical rejection capability of the zinc-rich mushrooms ethanol extract  is highest which is up to 392.79 (U / ml).

Key words  lentinus edodes  zinc-rich   antioxidation  Organic zinc