摘要：目的：以蟾毒靈為模型藥物，聚氰基丙烯酸正丁酯（PBCA）為載體，制備蟾毒靈PBCA納米粒，并對制備工藝進行篩選。方法：采用乳液聚合法，界面縮聚法，預(yù)合成PBCA法，乙醇法等方法制備蟾毒靈PBCA納米粒。以包封率和載藥量為考察指標，選擇最佳制備方法，并通過單因素試驗優(yōu)化制備工藝。結(jié)果表明：由乙醇法制得的納米粒，大小較為均勻，平均粒徑為149.2nm，多分散系數(shù)為0.258,包封率為75.751%，載藥量為5.601%。結(jié)論：本實驗優(yōu)化的乙醇法制備工藝穩(wěn)定可行。

關(guān)鍵詞：蟾毒靈；α-氰基丙烯酸正丁酯；納米粒；乙醇法

Abstract  objective：Using bufalin as the model drug, Poly n-butyl cyanoacrylate (PBCA) as the carrier, the bufalin PBCA nanoparticles was prepared and the preparation technology was optimized. Methods: Emulsion polymerization, interfacial polymerization, Pre synthesized PBCA, and ethanol method was used to prepare bufalin PBCA nanoparticles. The encapsulation efficiency and drug loading as investigated. The preparation and formulation was optimaized by single factor experiment. Results :Nanoparticles prepared by anhydrous ethanol has uniform size, the average particle size was 149.2nm, the polydispersity index was 0.258, encapsulation efficiency was 75.751%, drug loading was 5.601%. Conclusion: The optimum ethanol preparation process is stable and feasible.

Keywords:  bufaline; BCA; nanoparticles; ethanol method