摘要：隨著世界人口的逐漸老齡化, 老年性疾患也日見增多。本實驗主要針對由腦變性引起的阿爾茨海默癥（AD）進行展開，而Aβ在AD 的發病中起核心作用的假說已得到大多數人的認可。Aβ聚集可形成具有神經毒性的原纖維, 并進而形成AD的病理特征之一——老年斑。Aβ是由體內一種稱為淀粉樣蛋白前體蛋白（APP）經兩種分泌酶加工產生，即β-分泌酶和γ-分泌酶。其中β-分泌酶的活性是APP產生Aβ的關鍵酶。

　　　本實驗通過高效液相色譜初步建立了一種比較直觀的體外分析β-分泌酶活性的方法，建立出了酶活力動態曲線，并利用該方法為進一步分析阿爾茨海默氏癥（AD）的致病機理及篩選相應的生物活性物質打下基礎。在了解了β-分泌酶基本反應特性的基礎上，我們以β-分泌酶抑制劑為陽性對照物驗證了本分析方法的可行性。0.6um可達到34.43%的抑制程度，并隨著濃度的升高，其對β-分泌酶活性所起的抑制作用越明顯，呈現出了一定的線性關系。利用本評價方法，我們進一步測試了具有抗Aβ活性的姜黃素對β-分泌酶活性是否也有抑制作用，10um姜黃素即可達到17.80%的抑制效果。

關鍵詞：β-分泌酶， 阿爾茨海默癥（AD），β樣淀粉蛋白（Aβ）

Abstract：As the world's population is aging, senile disease also became more and more increased. This experiment mainly caused by degenerative brain of Alzheimer's disease (AD)，and Aβ in AD playing the core function of most people who have got the hypothesis recognition. Aβ gathered can form in the neurotoxicity of the fibers, senile plaque, as one of the pathological characteristic of AD. The Aβ in vivo is called amyloid precursor protein (APP), which is by two Secrete enzymes processing produced, the beta secrete enzymes (N end), and γ-secrete enzymes (C end). Beta secretion of the enzyme’s activity is the key enzymes in APP.

　　　This experiment is proposed by high performance liquid chromatography (HPLC), setting up a more intuitive analysis method of beta secrete enzymes. Through this analysis method, it may affect the factor of beta secretion enzyme activity for further analysis Alzheimer's disease (AD) pathogenesis mechanism and lay the foundation of corresponding screening of the biological activity material. By understanding the beta secretion basic response characteristics, we use beta secretion inhibitors for positive counterpart to this analysis, in order to verify the feasibility of the method. The beta secretion inhibitors of 0.6 um can reach 34.43% of the Inhibition degree. With the concentration increasing, the beta secretion of enzyme activity demonstrate the more obvious inhibition, presenting a certain linear relationship. Using this evaluation method, we further test the curcumin which is whether or not can resist to the activity of Aβ and have inhibition effect. The experimental results show that 10 um curcumin inhibitory effect can reach 17.80%.

Key Words: Beta secrete enzymes, Alzheimer's disease, β sample starch proteins (Aβ)