摘  要：從長期受石油污染的土壤中，分離出一株能高效降解石油的菌株ST-2，該菌株能以石油為唯一碳源生長。通過菌落及菌株形態(tài)，革蘭氏染色及穿刺實驗結(jié)果和生長曲線，查詢《伯杰細菌鑒定手冊》鑒定菌株ST-2屬葡萄桿菌屬。其生物學(xué)特性研究結(jié)果表明，ST-2菌株的生長對數(shù)期在2~24h，60h后進入衰亡期；最佳生長溫度為37oC，最佳生長pH為6.4，最佳鹽度為15g/L，在30-40oC，pH5.8-7.2，鹽度10-25 g/L之間都能良好生長；ST-2菌株的最適碳源為蔗糖和牛肉膏，也能很好的利用可溶性淀粉、葡萄糖；該菌株對牛肉膏作氮源的利用很好，無機氮源中對氯化銨的利用最好。

關(guān)鍵詞：石油污染，生物修復(fù)，ST-2菌株，影響因素，生物學(xué)特性

Abstract: Isolated from the long-term petroleum contaminated soil, one could effectively degrade the oil strain ST-2, the strains were able to to oil as the sole carbon source. Through the colony and strain morphology, Gram stain and puncture experimental results and the growth curve, the query "Berger bacterial identification manual identification of strain ST-2 is a grape spp. Their biological properties The results show that the growth of the ST-2 strain logarithmic phase in 2 ~ 24h, 60h and enter the decline phase; an optimum temperature of 37oC, the optimal growth pH of 6.4 and the optimum salinity of 15g / Lin 30-40oC, pH 5.8-7.2, salinity 10-25 g / L, can grow well; ST-2 strains, the optimal carbon source was sucrose and beef extract, but also make good use of soluble starch, glucose; the strains of beef extract as nitrogen source, the use of good, and inorganic nitrogen sources of ammonium chloride using the best.

Keywords: oil pollution, bioremediation, the ST-2 strains, influencing factors, biological characteristics