摘  要：從江蘇省常州市武進區、新北區、鐘樓區以及天寧區4個不同區域的污染場地采集污染土壤表層樣品A、B、C、D，通過在培養基中加入一定濃度的Cu（CuSO4 Cu2+2mmol·L-1），篩選得到3株具有很強Cu抗性的細菌ZH-1，ZH-2和ZH-3。對這些菌株生長特性和最低抑菌濃度對比進行分析，這三株菌的最低抑菌濃度分別為15、20和25 mmol/L。通過研究對這3種菌進行不同梯度濃度Cu環境中的生長規律，做出生長曲線，結果表明Cu在低濃度時對細菌生長幾乎沒什么影響，有的有輕微促進作用；隨著Cu濃度變大，對細菌有抑制作用。通過原子吸收分光光度計測定在5 mmol/LCu濃度下細菌對Cu的吸附能力，結果表明在12~24h吸附量最大，pH都遵循先降低后升高的規律。由于這些菌株分離于重金屬污染較為嚴重的土壤樣品中，從而對環境中Cu污染的微生物修復有一定的利用價值。

關鍵詞：銅；銅抗性菌；分離篩選；生長曲線；吸附能力

Abstract：From Wujin District,Xinbei District, the Clock Tower District,Tianning District,  samples were collected 4 soil samples A, B, and C and D, we isolated 3 strains of bacteria that are highly resistable for Copper , named after ZH-1、ZH-2 and ZH-3, making use of culture medium that contained Cu( CuSO4,Cu2+2mmol/L). These growth characteristics and the comparison of the minimum inhibitory concentration, minimum inhibitory concentration of the three bacteria were 15, 20 and 25 mmol / L. Different gradient concentrations of Cu in the growth of law through the study of these three kinds of bacteria, the results show that Cu at low concentrations, little effect on bacterial growth, and some have a minor role; with the larger Cu concentration, bacteria have inhibitory role. Spectrophotometer in 5 mmol/LCu bacteria concentrations of Cu absorbance (adsorption capacity), the results show that the maximum adsorption capacity in 12~24h, and it follows the law that pH declines firstly and grows then. These strains were isolated in the more serious heavy metal contamination in soil samples, which Cu contamination of microorganisms in the environment to repair a certain use value.

Key words: Copper; Copper-resistant bacteria; Separation filter; Growth curve; Adsorption