摘要:在單因素的基礎上建立了一個以多糖得率為目標值，以液料比、酶濃度和酶解溫度為因素的數學模型，方差分析表明擬合較好。通過對回歸方程優化計算，得到提取的最佳工藝條件為液料比11.4mL/g、酶濃度0.43%和酶解溫度45.4℃。對所建立的數學模型進行了試驗驗證。在最佳工藝條件下，得到多糖的得率為5.33%，與理論值5.28%基本一致。白靈菇蘆薈飲料的最佳配方為配比1:2、混合液所占飲料比1:10、白砂糖添加量10%和檸檬酸添加量0.12%，此時產品綜合品質最優。

關鍵詞：白靈菇；蘆薈；多糖；復合保健飲料

Abstract:The influences of solvent to material ratio, enzyme concentration and enzymolysis temperature on the yield of the polysaccharide from pleurotus nebrodensis with the cellulase-assisted extraction technique were studied according to the Box-Behnken experimental design based on the single factor experiments. One mathematical model established and analyzed by response surface methodology was adequate to describe the relationships between the studied factors and the response of the polysaccharide extraction yield. Based on the canonical analysis, the optimum extraction conditions were obtained as follows: solvent to material ratio 11.4mL/g, enzyme concentration 0.43% and enzymolysis temperature 45.4℃. Under the optimized extraction conditions, it can be included that the extraction yield is 5.33%, which is close to the estimated value 5.28% attained by using regression model. This model can be used to predict the experiment results well. The best recipe of compound beverage of pleurotus nebrodensis and aloe was volume of pleurotus nebrodensis to volume of aloe 1:2, mass of mixed liquor to mass of total beverage 1:10, sucrose amount 10% and citric acid amount 0.12%.

Keywords: Pleurotus Nebrodensis; Aloe; Polysaccharides; Compound health beverage