摘要:在單因素的基礎上建立了一個以吸光度為目標值，以液料比、酶解溫度和酶解時間為因素的數學模型，方差分析表明擬合較好。通過對回歸方程優化計算，得到提取的最佳工藝條件為液料比15.7mL/g、酶解溫度49.5℃和酶解時間54.9min，此時吸光值達到最大值0.641，與理論值0.634基本一致。紫羅蘭白靈菇復合保健飲料的最佳配方為配比2:1、混合液所占飲料比1:10、白砂糖添加量10%和檸檬酸添加量0.12%；紫羅蘭雙孢菇復合保健飲料的最佳配方為配比2:1、混合液所占飲料比1:10、白砂糖添加量10%、蜂蜜添加量0.6%和檸檬酸添加量0.10%；紫羅蘭蟹味菇復合保健飲料的最佳配方為配比2:1、混合液所占飲料比1:10、白砂糖添加量10%、蜂蜜添加量0.6%和檸檬酸添加量0.10%。

關鍵詞：紫羅蘭；白靈菇；雙孢菇；蟹味菇；復合保健飲料

Abstract:The influences of solvent to material ratio, enzymolysis temperature and enzymolysis time on the absorbance of pigment of Matthiola Incana. With the cellulase-assisted extraction technique were studied according to the Box-Behnken experimental design based on the single factor experiments. One mathematical model established and analyzed by response surface methodology was adequate to describe the relationships between the studied factors and the response of pigment absorbance. Based on the canonical analysis, the optimum extraction conditions were obtained as follows: solvent to material ratio 15.7mL/g, enzymolysis temperature 49.5℃ and enzymolysis time 54.9min. Under the optimized extraction conditions, it can be included that the absorbance is 0.641, which is close to the estimated value 0.634 attained by using regression model. This model can be used to predict the experiment results well. The best recipe of compound health beverage of Matthiola Incana and Pleurotus Nebrodensis was volume of Matthiola Incana to volume of Pleurotus Nebrodensis 2:1, mass of mixed liquor to mass of total beverage 1:10, sucrose amount 10% and citric acid amount 0.12%; the best recipe of compound health beverage of Matthiola Incana and Agaricus Bisporus was volume of Matthiola Incana to volume of Agaricus Bisporus 2:1, mass of mixed liquor to mass of total beverage 1:10, sucrose amount 10%, honey amount 0.6% and citric acid amount 0.10%; the best recipe of compound health beverage of Matthiola Incana and Hypsizygus Marmoreus was volume of Matthiola Incana to volume of Hypsizygus Marmoreus 2:1, mass of mixed liquor to mass of total beverage 1:10, sucrose amount 10%, honey amount 0.6% and citric acid amount 0.10%.  

Keywords: Matthiola Incana; Pleurotus Nebrodensis; Agaricus Bisporus; Hypsizygus Marmoreus; Compound health drink