摘要:本實驗以原種水稻WT和轉PEPC基因水稻為材料，研究NaHSO3對轉PEPC基因水稻光合生理特性的影響。測定了PEPC酶活性和葉綠素含量，外源飼喂低濃度的NaHSO3后轉PEPC基因水稻葉片光合速率，ATP的變化，結果顯示，轉PEPC基因水稻PEPC酶活性和葉綠素含量比原種高， NaHSO3 處理后,可顯著提高轉PEPC基因水稻的光合速率和ATP含量，說明NaHSO3是轉PEPC基因水稻光和生理過程的限制因素，為進一步構建類似C4水稻提供理論依據。

關鍵詞：轉PEPC基因水稻，NaHSO3，光合速率。

Abstract:With untransformed rice and PEPC transgenic rice as the materials, the activity of PEPC enzymes, chlorophyll contents, effect of NaHSO3 on Photosynthesis Characteristic and ATP content of the PEPC gene rice were studied in this paper.The results showed that the activity of PEPC enzyme and chlorophyll contents in PEPC transgenic rice were higher than that in wild type , After treatment with NaHSO3 ,the photosynthetic rate and ATP content were significantly increased in the PEPC transgenic rice. This indicated that ATP is the key limited factor for constructing C4 like rice, providing a new technical approach to constructing C4 like rice.

Key words: PEPC gene rice, NaHSO3, photosynthesis rates.