摘  要

　　　本實(shí)驗(yàn)以牛蛙（Rana catesbiana）為材料，用氯化鉛配制不同濃度的Pb2+溶液（10、20和40 mg/kg）。在染毒7天后，對(duì)牛蛙肌肉和神經(jīng)組織興奮閾值、總蛋白質(zhì)含量、丙二醛（Malondialdehyde, MDA）含量、過氧化氫酶（Catalase, CAT）活性以及重金屬Pb2+的殘留量進(jìn)行測定，探討分析實(shí)驗(yàn)結(jié)果。實(shí)驗(yàn)結(jié)果表明，處理前牛蛙呼吸頻率無顯著差異，處理后呼吸頻率有顯著差異，心率隨濃度的增大而降低。牛蛙肌肉和神經(jīng)組織興奮閾值隨重金屬Pb2+離子濃度的增大而增大，總蛋白質(zhì)含量、MDA含量隨濃度的增大而升高，CAT活性隨濃度的增大而降低，在腹肌、后腿肌肉和肝臟中重金屬Pb2+的殘留量最多的是肝臟，其次是腹肌，最少的是后腿肌肉。

關(guān)鍵詞：牛蛙；鉛；組織興奮閾值；總蛋白質(zhì)；丙二醛；過氧化氫酶

ABSTRACT

　　　In this experiment to Bullfrogs (Rana catesbiana) for materials, prepared with lead chloride solution of different concentration in Pb2+ (10, 20 and 40 mg/kg). After the 7 day exposed, Rana catesbiana muscle and nerve tissue exciting threshold, total protein, malondialdehyde (Malondialdehyde, MDA) level, catalase (Catalase, CAT) activity and determination of residue of heavy metal Pb2+, discussion on the analysis of experimental results. The results showed that former Rana catesbiana no significant difference in respiratory rate, respiratory frequency differences after treatment, heart rate decrease with the increase in concentration. Muscle and nerve tissue excitatory threshold value with the Pb2+ ion of heavy metal concentrations increase and increase. Total protein content, MDA content increased with increasing concentration, CAT activity decreased with the increase of the concentration. In abdominal, back leg muscles and liver are liver Pb2+ residues of heavy metals in the most, followed by abdominal muscle, the least is hind leg muscles.

Key words: Rana catesbiana; Lead; Organization excitation threshold; Total proteins; Malondialdehyde; Catalase