摘要:以銀杏粉為原料，以高效液相色譜(HPLC)測定分配系數的方法優選高速逆流色譜(HSCCC)分離銀杏黃酮的最佳溶劑體系，探討高速逆流色譜儀的流動相流速、轉速、分離溫度等因素對銀杏黃酮成分分離的影響。最終方案確定了最佳的HSCCC分離條件：溶劑體系為正己烷-乙酸乙酯-甲醇-水(4.5:5.5:5:5，v/v)，流速2 mL/min、轉速800 r/min、分離溫度25 °C，經HSCCC，在銀杏葉提取物中分離得到三種黃酮類化合物，經HPLC分析為槲皮素、山奈酚、異鼠李素，純度都達到90%以上。

　　　對分離得到的槲皮素、山奈酚和異鼠李素進行抗氧化性分析，結果表明具有一定的清除羥基自由基和DPPH自由基能力，清除能力與濃度呈較明顯的量效關系；穩定性分析實驗表明，銀杏黃酮類化合物對溫度、時間和光照等具有較好的穩定性，適用于食品生產。

關鍵詞 銀杏黃酮；高速逆流色譜分離；槲皮素；山奈酚；異鼠李素；抗氧化性；穩定性

Abstract:Ginkgo powder as raw material, measured by high performance liquid chromatography (HPLC) method of distribution coefficient is preferably high-speed countercurrent chromatography (HSCCC) separation solvent system to explore the high-speed counter-current chromatography mobile phase flow rate, speed, separation temperature and other factors of Ginkgo flavonoids isolated. Determining the optimum HSCCC separating conditions: solvent system of n-hexane - ethyl acetate - methanol - water (4.5:5.5:5:5, v/v), flow rate of 2 mL/min, the rotational speed of 800r/min, separation temperature 25 °C, HSCCC, Ginkgo biloba extract isolated components of three flavonoids, quercetin, kaempferol, isorhamnetin, purity by HPLC analysis was more than 90%. 

　　　Isolated quercetin, kaempferol and isorhamnetin oxidation resistance analysis results show that the scavenging DPPH radical scavenging ability and concentration showed an obvious dose-effect relationship; stability The experiments show that, the Ginkgo flavonoids has good stability under alkaline conditions of temperature, time and light, suitable for food production.

Keywords  Ginkgo flavonoids High-speed counter current chromatography Quercetin Kaempferol  Isorhamnetin Antioxidant activity  Stability