摘要:研究不同有機試劑萃取牛蒡乙醇提物的抗氧化性能，選擇最優的進行分離純化后研究各組份抗氧化性能,并對其有效成分結構做相應鑒定。牛蒡皮醇提物分別用石油醚、乙酸乙酯、正丁醇三種有機溶劑萃取，提取物采用ORAC法研究其抗氧化性，對最優組分采用高速逆流及高效液相色譜法進行分離純化。四種牛蒡提取物中，乙酸乙酯萃取相的抗氧化性能最優；將乙酸乙酯提取物通過高速逆流進一步分離純化，得出高速逆流收集相A的抗氧化性能優于其收集相B；對收集相A進行高效液相色譜分析，初步得出收集相A含有咖啡酸、綠原酸等多種成分，今后我們將對這些化合物做進一步的分析研究，并對其做結構鑒定。

關鍵詞 牛蒡；乙醇提取物；抗氧化；分離純化

Abstract:To study the different organic solvents extraction burdock ethanol-extraction content antioxidant properties, choose the best separation and purification study after the components oxidation resistance. And makes corresponding identification of the effective component structure. Burdock skin ethanol-extraction respectively with petroleum ether, ethyl acetate, n-buoh extraction, made by ORAC to find out the effect of remove oxidation resistance, separation and purification of the optimal components by HSCCC and HPLC. Burdock leather alcohol, petroleum ether, ethyl acetate, n-butanol extract, Burdock skin acetic ester extract oxidation resistance is the best. The ethyl acetate extract by HSCCC further purification, HSCCC drawn A antioxidant properties superior to its collection of B. To collect A HPLC analysis, preliminary concluded that collect drawn A caffeic acid, chlorogenic acid and other ingredients, in the future we will do further analysis and research of these compounds, and the structure identification.

Keywords  Burdock  ethanol-extraction  antioxidant  separation and purification