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摘 要
本試驗(yàn)以白花蛇舌草作為供試材料，采用KT、2,4-D、蔗糖，三物質(zhì)不同濃度和光質(zhì)配比處理，通過四因素三水平正交試驗(yàn)設(shè)計(jì)，以篩選出適合白花蛇舌草愈傷組織繼代培養(yǎng)的最佳配方，然后以最佳配方為基礎(chǔ)，采用L9（34）試驗(yàn)設(shè)計(jì)He-Ne激光不同輻射劑量、不同輻射時(shí)間及不同光照強(qiáng)度的配比處理，通過對吲哚乙酸氧化酶活性等生理生化指標(biāo)測定，篩選出有利于白花蛇舌草愈傷組織生長的最優(yōu)組合。試驗(yàn)結(jié)果表明：在正常光培養(yǎng)條件下，白花蛇舌草的繼代培養(yǎng)的最佳配方是MS+2,4-D4.0mg/L+KT1.5mg/L+蔗糖30g/L。在He-Ne激光輻射時(shí)間與劑量分別為3mA、3min和光照強(qiáng)度為1500lx時(shí)，對愈傷組織的吲哚乙酸氧化酶活性的影響達(dá)到最低；在He-Ne激光輻射時(shí)間與劑量分別為6mA、3min和光照強(qiáng)度為2000lx時(shí)，對愈傷組織的可溶性蛋白質(zhì)含量的影響達(dá)到最大；在He-Ne激光輻射時(shí)間與劑量分別為9mA、3min和光照強(qiáng)度為2500lx時(shí)，對可溶性糖含量的影響達(dá)到最大。其中He-Ne激光輻射時(shí)間與劑量分別為3mA、3min和光照強(qiáng)度為1500lx時(shí)，為最佳組合。
關(guān)鍵詞：白花蛇舌草；愈傷組織；繼代培養(yǎng)；He-Ne激光
ABSTRACT
This experiment with Hedyotis diffusa willd as selected materials, Using KT、2,4-D、sucrose,Three different concentrations and light qualitative material ratio processing , Through four factors of three levels orthogonal experiment design, To screened for Hedyotis diffusa willd the best prescription of callus subculture, Then based on the best formula, Adopt L9 (34) experiment design He-Ne laser different radiation dose、different time and different illumination intensity radiation ratio of processing, Based on such indoles acetic acid enzymatic activity determination of physiological indexes, Screened out callus growth is conducive to the optimal combination of Hedyotis diffusa Willd. The results show that: Culture conditions in normal light, Hedyotis diffusa Willd subculture best formula is MS +2,4-D4 .0 mg / L + KT1.5mg / L + sucrose 30g / L. In the He-Ne laser irradiation time and dose of 3mA, 3min, and when the light intensity was 1500lx, Indole acetic acid callus of enzyme activities to meet the minimum;In the He-Ne laser irradiation time and dose of 6mA, 3min, and when the light intensity was 2000lx, For callus soluble protein content reached the maximum; In the He-Ne laser irradiation time and dose were 9mA, 3min, and when the light intensity was 2500lx, the maximum of Soluble sugar content. Which He-Ne laser irradiation time and dose of 3mA, 3min, and when the light intensity was 1500lx, For the best combination.
Key words: Hedyotis diffusa willd; Callus; Subculture; He-Ne laser
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