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      稻曲球中主要稻曲菌素的分離和純化_生物技術(shù).doc

      資料分類(lèi):農(nóng)業(yè)大學(xué) 上傳會(huì)員:淺淺的回憶 更新時(shí)間:2013-06-21
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      摘要

         稻曲病是由稻曲菌感染所引起的水稻真菌病害。稻曲菌素是稻曲菌的次生代謝產(chǎn)物之一,為水溶性物質(zhì)。研究發(fā)現(xiàn)稻曲菌素是一類(lèi)環(huán)肽毒素,能夠抑制真核生物微管蛋白的組裝與細(xì)胞骨架的形成、抑制細(xì)胞有絲分裂,從而表現(xiàn)出明顯的細(xì)胞毒活性,對(duì)動(dòng)物和植物產(chǎn)生毒害作用。稻曲菌素在抗腫瘤、抗真菌和殺蟲(chóng)等方面具有廣泛的農(nóng)藥和醫(yī)藥用途。

         本論文工作對(duì)稻曲菌素的分離和純化條件進(jìn)行摸索。首先通過(guò)對(duì)采集的稻曲球進(jìn)行甲醇的浸泡和蒸發(fā)濃縮而得到稻曲菌素的粗提物。然后經(jīng)過(guò)石油醚、乙酸乙酯及正丁醇的萃取得到石油醚層、乙酸乙酯層和正丁醇層。經(jīng)點(diǎn)板后分析稻曲菌素在正丁醇層含量較高。乙酸乙酯層通過(guò)切割柱后,合并為8個(gè)部分。正丁醇層經(jīng)過(guò)三種大孔吸附樹(shù)脂預(yù)實(shí)驗(yàn)后,發(fā)現(xiàn)大孔吸附樹(shù)脂HP-20乙醇梯度洗脫法效果較好。研究結(jié)果為后續(xù)分離和純化稻曲菌素提供了依據(jù)。 

      關(guān)鍵詞:稻曲病; 稻曲病菌;稻曲菌素; 分離; 純化

       

      Abstract

         Rice false smut is caused by the infection of the fungal pathogen Ustilaginoidea virens. Ustiloxins are water-soluble substances, which are the secondary metabolites produced by U. virens. Study found that ustiloxins are cyclopeptide mycotoxins. They are toxic to plants and animals especially for their cytotoxic activity to be antimitotic by inhibition of microtubule assembly and cell skeleton formation. Ustiloxins can be used for medicinal and agrochemical purposes acting as antitumor, antifungal and insecticidal agents. 

         This experiment explored the conditions of isolation and purification of ustiloxins. We obtained crude ustiloxins through the way that we socked the collected false smut balls in methanol and then evaporation the extracts. After the extraction of petroleum ether, ethyl acetate and normal butyl alcohol, we got petroleum ether level, ethyl acetate level and normal butyl alcohol level. Compared with ethyl acetate level, the quantity of ustiloxins was much higher in the normal butyl alcohol level after thin layer chromatographic analysis. Ustiloxins in the ethyl acetate level merge into eight parts after the cutting column. We made a pre-experiment about normal butyl alcohol level through three kinds of macfofeticulaf resins, and to find the method that macroporous resin HP-20 eluted by different concentrations of ethanol is better. These research results can make a basement for the next isolation and purification of ustiloxins.

      Key Words:Rice false smut; Ustilaginoidea virens; ustiloxins; isolation; purification

       

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      上傳會(huì)員 淺淺的回憶 對(duì)本文的描述:本論文工作對(duì)稻曲菌素的分離和純化條件進(jìn)行摸索。首先通過(guò)對(duì)采集的稻曲球進(jìn)行甲醇的浸泡和蒸發(fā)濃縮而得到稻曲菌素的粗提物。然后經(jīng)過(guò)石油醚、乙酸乙酯及正丁醇的萃取得到石油......
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