摘要:本實(shí)驗(yàn)采用復(fù)合酶法研究了牛蒡中多糖的提取工藝條件，采用Sevag法脫蛋白分離純化牛蒡多糖，采用薄層層析法研究其組成分。實(shí)驗(yàn)結(jié)果表明：復(fù)合酶用量最佳配比為酸性纖維素酶3.0%、糖化酶2.0%、酸性蛋白酶3.5%，此條件下的牛蒡多糖提取率為7.49%；復(fù)合酶法提取最佳工藝條件為料液比1:20、提取溫度45℃、提取時(shí)間60min，此條件下的牛蒡多糖提取率為7.64%；用Sevag法純化粗多糖，純度提高了35.10%；通過(guò)薄層層析，確定最佳水解酸為硫酸，最佳展開(kāi)系統(tǒng)為正丁醇：乙酸：水= 4 :1: 5，牛蒡多糖由葡萄糖、半乳糖、果糖組成。

關(guān)鍵詞 牛蒡；多糖；復(fù)合酶；分離純化；薄層層析

Abstract:This experiment adopted complex enzymatic the burdock extract polysaccharide in process conditions, The Sevag method to take off the protein purification burdock polysaccharide, and the thin layer chromatography study its constituent. The experimental results showed that: the best ratios for composite enzyme dosage were that cellulose enzyme was 3%, that saccharifying enzyme was 2%, and that acid protease was 3.5%, the conditions of the burdock polysaccharide extraction yield was 7.49%. The best enzymolysis conditions forcomposite enzymatic extraction were that feed liquid ratio was 1:20, that extraction temperature was 45℃, and that extraction time was 60min. the conditions of the burdock polysaccharide extraction yield was 7.64%. Sevag method with purified crude polysaccharide, purity increased by 35.10%. By thin layer chromatography, to determine the optimal hydrolysis acid was sulfuric acid, the best expand system was n-butanol: acetic acid: water = 4: 1: 5, burdock polysaccharide consisted of glucose, galactose, fructose.

Keywords  Burdock    Polysaccharide    Composite enzyme    Separation and purification     Thin-layer chromatography