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摘 要:本文主要包括兩個部分,綜述部分和實驗部分。 第一部分首先對銀蓮屬植物化學成分研究情況進行綜述,根據資料調研得出,在該屬植物的研究中發現了皂苷、內酯類、香豆素、揮發油、油脂、氨基酸、微量元素及其他一些成分;其次對其藥理作用進行了闡述,該屬植物有具抗癌、抗炎、抗菌、鎮痛、鎮靜等作用,因其廣泛的藥理作用,該屬植物在未來具有廣闊的發展前景。 第二部分為實驗部分,主要敘述了實驗的具體流程,對其具體步驟進行簡要的描述,對實驗中的部分問題提出了自己的看法。此次試驗對云南省紅河州藥用植物野棉花的化學成分進行了研究,實驗中采用硅膠柱層析、梯度洗脫法對野棉花氯仿部分的化學成分進行分離純化研究,運用薄層層析法定性鑒別,進一步分離純化,根據TLC的結果初步判斷得到2個化合物。 關鍵詞:銀蓮屬;野棉花;化學成分;柱層析;薄層層析法
ABSTRACT:This article mainly includes two parts, Review part and experimental section. The first part of Anemone first chemical constituents were reviewed, according to the situation of the research, the conclusion in data study of the genus discovered saponins, lactone class, coumarin, naphtha, grease, amino acids, trace elements and some other component; Second on the pharmacological effects were introduced, these plants there a anti-cancer, anti-inflammatory, antibacterial, analgesia, calm effects, and because of its extensive pharmacological effects in the future, this genus has wide development prospects. The second part is, experimental section mainly described the concrete process of experiment, the specific steps to brief description, Part of the problem for experiments proposed own view. The test of yunnan honghe medicinal plants Anemone vitifola Buch.-Ham, chemical experiments were studied by silica column chromatography, gradient elution method to Anemone vitifola Buch.-Ham chloroform part of the chemical composition, use TLC to identificate of qualitativation, further purification. According to the result of TLC judge get 2 compounds . Keywords: Anemone vitifola Buch.-Ham; Chemical composition; Chromatography; TLC |